CST, 87537S, 3X DYKDDDDK Tag (E7C5T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, 3X DYKDDDDK Tag (E7C5T) Rabbit Monoclonal Antibody (CST #87537) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity 3X DYKDDDDK Tag (E7C5T) Rabbit Monoclonal Antibody detects exogenously expressed proteins containing the 3X DYKDDDDK epitope tag. This antibody recognizes the 3X DYKDDDDK tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells, but does not detect DYKDDDDK tag. This antibody detects exogenously expressed 3X DYKDDDDK-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the 3X DYKDDDDK epitope (DYKDHDGDYKDHDIDYKDDDDK). Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG