CST, 87538T, METTL16 (E2J1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying METTL16. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, METTL16 (E2J1D) Rabbit Monoclonal Antibody (CST #87538) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity METTL16 (E2J1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total METTL16 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala9 of human METTL16 protein. Background METTL16 is an N6-adenosine methyltransferase responsible for the regulation of the gene, which encodes S-adenosylmethionine (SAM) synthase. Upon SAM depletion, MAT2A expression increases due to a splicing event of a retained intron. Alternative splicing and mRNA stability is governed by adenosine methylation in the 3' UTR of the MAT2A mRNA by METTL16. These marks are then read by YTHDC1, and knockdown of either METTL16 or YTHDC1 results in decreased response to lack of SAM (1,2). The METTL16 methyltransferase domain differs from METTL3 and METTL14 as it contains an extra N-terminal module, suggesting a different set of target mRNAs (3,4). Lack of METTL16 during development has been shown to be embryonically lethal, resulting in a dysregulated transcriptome that cannot proceed past the 64-cell blastocyst stage (3). Alternate Names MALAT1; MET10; MET16; methyltransferase 10 domain containing; Methyltransferase 10 domain-containing protein; methyltransferase like 16; Methyltransferase-like protein 16; METT10D; METTL16; MGC3329; N6-adenosine-methyltransferase METTL16; Putative methyltransferase METT10D; RNA N6-adenosine-methyltransferase METTL16; U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase; U6 snRNA methyltransferase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG