CST, 8756S, BIRC6 (D8B5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BIRC6. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, BIRC6 (D8B5) Rabbit Monoclonal Antibody (CST #8756) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BIRC6 (D8B5) Rabbit Monoclonal Antibody recognizes endogenous levels of total BIRC6 protein. Several bands of unknown origin between 120 and 500 kDa are detected in some cell lines. It is possible that these bands are degradation products of BIRC6. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp147 in human BIRC6 protein. Background BIRC6/BRUCE/APOLLON is a member of the inhibitor of apoptosis protein (IAP) family. BIRC6 is a huge 530 kDa membrane-associated protein with a single survivin-like baculoviral IAP repeat (BIR) domain at the amino terminus, and a ubiquitin-conjugating enzyme domain at the carboxy terminus (1-3). Several research studies support the notion that BIRC6 functions as a dual regulator of cell death and cell division (4-6), and BIRC6 is the only essential BIR-containing protein in mammalian cell growth and development (4,7). Research studies have documented the overexpression of BIRC6 in colon cancer stem cells and in other cancer cell lines (8,9). BIRC6 inhibits apoptosis by either 1) binding to and inhibiting caspases (10) or 2) ubiquitinating the IAP antagonist SMAC and the apoptosis initiator caspase 9, thereby targeting these proteins for proteasomal degradation (4,5). BIRC6 itself is regulated by ubiquitination and proteasomal degradation upon stimulation of apoptosis (7,11). Alternate Names APOLLON; baculoviral IAP repeat containing 6; baculoviral IAP repeat-containing 6; Baculoviral IAP repeat-containing protein 6; BIR repeat-containing ubiquitin-conjugating enzyme; BIRC6; BRUCE; FLJ13726; FLJ13786; KIAA1289; RING-type E3 ubiquitin transferase BIRC6; Ubiquitin-conjugating BIR domain enzyme apollon; ubiquitin-conjugating BIR-domain enzyme apollon Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 530 Source/Isotype: Rabbit IgG