CST, 87572T, CD300a (E6B9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD300a. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD300a (E6B9T) Rabbit Monoclonal Antibody (CST #87572) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD300a (E6B9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD300a protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala210 of human CD300a protein. Background CD300a is an inhibitory receptor in the Ig superfamily, a type I transmembrane protein containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) on its cytoplasmic tail (1,2,5). Human CD300a (CMRF-35H, IRp60) and mouse CD300a (CLM-8, LMIR-1, MAIR-I) are functional orthologs, expressed by monocytes, granulocytes, mast cells, and subsets of B cells (1-5). Human, but not mouse, CD300a is also expressed by unstimulated NK cells and subsets of T cells (1,5). Phosphorylated ITIMs are able to recruit different phosphatases, such as SHP-1 and SHP-2, leading to inhibition of cell activation (1,5). Alternate Names CD300 antigen-like family member A; CD300A; CD300a antigen; CD300a molecule; CLM-8; CLM8; CMRF-35-H9; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF35-like molecule 8; CMRF35H; CMRF35H leukocyte immunoglobulin-like receptor; CMRF35H9; IGSF12; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; MAIR-I; NK inhibitory receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-70 Source/Isotype: Rabbit IgG