CST, 87661SF, NT5E/CD73 (D7F9A) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NT5E. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, NT5E/CD73 (D7F9A) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #87661) is ready to ship. Product Usage Information This product is the carrier free version of product #13160. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 13160 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NT5E/CD73 (D7F9A) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NT5E/CD73 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human NT5E/CD73 protein. Background Ecto-5'-nucleotidase (NT5E, also called CD73) is a 70 kDa glycosyl phosphatidylinositol-anchored, membrane-bound glycoprotein that catalyzes the hydrolysis of extracellular nucleoside monophosphates into bioactive nucleosides (1). NT5E catalyzes the terminal step of extracellular adenosine formation from adenosine monophosphate, which drives the regulation of extracellular adenosine levels and the downstream activation of the four G protein-coupled adenosine receptors (2). Binding of hypoxia-inducible factor (HIF-1) to the NT5E gene promoter leads to upregulation of NT5E during hypoxia (3). The biological roles of NT5E include lymphocyte adhesion (4,5), fibrosis (6), and the regulation of nociception (7,8). Alternate Names 5' nucleotidase (CD73); 5'-NT; 5'-nucleotidase; 5'-nucleotidase ecto; 5'-nucleotidase, ecto (CD73); 5NTD; CALJA; CD73; E5NT; Ecto-5'-nucleotidase; eN; eNT; NT; NT5; NT5E; NTE; Purine 5-Prime-Nucleotidase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG