CST, 87713S, IFNAR2 (E7Z4M) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying IFNAR2. Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, IFNAR2 (E7Z4M) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #87713) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity IFNAR2 (E7Z4M) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IFNAR2 protein. Background Interferon alpha/beta receptor 2 (IFNAR2) is one of the two protein subunits that comprise the type I interferon (IFN-I) surface receptor. IFNAR2 is a class II helical cytokine receptor with an ectodomain composed of two fibronectin type III-like subdomains, D1 and D2, and an unstructured intracellular domain (ICD) (1,2). IFNAR2 is expressed on most nucleated cells (3). IFN-Is bind the IFN receptor, leading to the dimerization of IFNAR2 and IFNAR1 subunits, and the activation of Janus family kinase (JAK) and signal transducer and activator of transcription (STAT) signaling pathways (4,5). JAK1 is associated with IFNAR2, and STAT1 and STAT2 are constitutively bound to the intracellular domain (ICD) of IFNAR2 (6,7). The formation of the transcription factor IFN-stimulated gene factor 3 (ISGF3), which is a complex made up of the pSTAT1-pSTAT2 heterodimer and interferon regulatory factor 9 (IRF-9), leads to the transcription of interferon-stimulated genes (ISGs) (8). IFN-I subtypes have differential binding affinity toward IFNAR2, contributing to differences in response potency (9). In contrast, IFN-αs all bind IFNAR1 with a low affinity and only IFN-β binds IFNAR1 with a relatively higher affinity (10). IFN-I signaling mediates various cellular responses, including antiviral responses, immunomodulatory responses, and antiproliferative effects (3). In the tumor microenvironment (TME), the presence of IFN-I signaling is associated with "hot" tumors in which key effector immune cells are present and is predictive of therapeutic response, whereas suppressed IFN-I signaling in the TME is associated with tumor progression and poorer outcomes (11,12). IFN-I signaling is being investigated as a therapeutic target for cancer, autoimmunity, sepsis, and viral infection (13,14). Alternate Names human interferon alpha/beta receptor; IFN-alpha binding protein; IFN-alpha-REC; IFN-alpha/beta receptor 2; IFN-R; IFN-R-2; IFNABR; IFNAR2; IFNARB; IMD45; INAR2; interferon (alpha, beta and omega) receptor 2; interferon alpha and beta receptor subunit 2; Interferon alpha binding protein; Interferon alpha/beta receptor 2; interferon receptor; interferon-alpha/beta receptor beta chain; Type I interferon receptor 2 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG