CST, 87716SF, Calretinin (E7R6O) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying calretinin/CALB2. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, Calretinin (E7R6O) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #87716) is ready to ship. Product Usage Information This product is the carrier free version of product #92635. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 92635 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Calretinin (E7R6O) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Calretinin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Calretinin protein. Background Calretinin (29 kDa calbindin, calbindin 2) is a calcium-binding protein of the EF-hand family encoded by the gene. It is differentially expressed from homologous family member calbindin-d28k in distinct neuronal populations of the retina, auditory system, and cerebellar granule cells (1,2), and acts as a marker for specific neuronal subsets of the subthalamic nucleus and the substantia nigra (3). Calretinin has been shown to play an important role in modulating neuronal excitability and the induction of long-term potentiation (1). Research has shown that, pathologically, Calretinin is a selective marker for epithelial mesothelioma, making it a diagnostic tool to differentiate from adenocarcinomas (4). Alternate Names 29 kDa calbindin; CAB29; CAL2; CALB2; calbindin 2; calbindin 2, (29kD, calretinin); calbindin D29K; Calretinin; CR; testicular secretory protein Li 8 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG