CST, 8774S, KIBRA Antibody

Polyclonal Antibody for studying KIBRA. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, KIBRA Antibody (CST #8774) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity KIBRA Antibody recognizes endogenous levels of total KIBRA protein. Higher molecular weight bands detected by western blot are phosphorylated forms of KIBRA. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala585 of human KIBRA protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of YAP (Yes-associated protein), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). Recent studies have identified KIBRA as a novel regulator of Hippo signaling (5-7). KIBRA has been shown to regulate Hippo signaling through its interaction with tumor suppressors Merlin (Mer) and Expanded (Ex) in (7) and by associating with large tumor suppressors LATS1 and LATS2 in humans (8). In humans, KIBRA is predominantly expressed in the kidney and brain (9) and has been shown to play a role in hippocampus-related memory performance (10-12). Recent studies have shown that phosphorylation of KIBRA is highest during mitosis and is controlled by aurora kinase and protein phosphatase 1 (13). Alternate Names FLJ10865; FLJ23369; HBeAg-binding protein 3; HBEBP3; HBEBP36; KIAA0869; KIBRA; Kidney and brain protein; MEMRYQTL; PPP1R168; Protein KIBRA; protein phosphatase 1, regulatory subunit 168; protein WWC1; WW and C2 domain containing 1; WW domain-containing protein 1; WW, C2 and coiled-coil domain containing 1; WWC1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 135 SOURCE: Rabbit