CST, 87781S, IFIT3 Antibody

Polyclonal Antibody for studying IFIT3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, IFIT3 Antibody (CST #87781) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IFIT3 Antibody recognizes endogenous levels of total IFIT3 protein. This antibody detects 110 kDa and 300 kDa bands of unknown origin in some cell lines. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro353 of human IFIT3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background IFIT3 (Interferon-induced protein with tetratricopeptide repeats 3; Retinoic acid-induced gene G protein (RIG-G)) is part of the IFIT family of interferon-stimulating genes known for their antiviral properties (1,2). IFIT3 is widely expressed in T cells, B cells, plasmacytoid and myeloid dendritic cells, neurons, and kidney cells (3,4). In response to RNA viral infection, IFIT3 regulates IRF3 activation through interaction with TBK1 and MAVS on the mitochondrion (5). IFIT3 also interacts with IFIT1, leading to inhibitory activity against viral RNA lacking the 2′-O-methylation in the cap structure (6). Alternate Names CIG-49; cig41; CIG49; GARG-49; IFI-60K; IFI60; IFIT-3; IFIT-4; IFIT3; IFIT4; interferon induced protein with tetratricopeptide repeats 3; Interferon-induced 60 kDa protein; Interferon-induced protein with tetratricopeptide repeats 3; Interferon-induced protein with tetratricopeptide repeats 4; IRG2; ISG-60; ISG60; P60; Retinoic acid-induced gene G protein; RIG-G Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-65 SOURCE: Rabbit