CST, 87786SF, LAMTOR1/C11orf59 (D11H6) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LAMTOR1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, LAMTOR1/C11orf59 (D11H6) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #87786) is ready to ship. Product Usage Information This product is the carrier free version of product #8975. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8975 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LAMTOR1/C11orf59 (D11H6) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total LAMTOR1/C11orf59 protein. This antibody may cross-react with LAMTOR2/ROBLD3 and LAMTOR3/MAPKSP1. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human LAMTOR1/C11orf59 protein. Background mTORC1 kinase complex is a critical component in the regulation of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids (3,4). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit the four Rag GTPases to the surface of lysosomes (5). Alternate Names C11orf59; chromosome 11 open reading frame 59; CK059; FLJ20625; LAMTOR1; Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1; late endosomal/lysosomal adaptor, MAPK and MTOR activator 1; Lipid raft adaptor protein p18; LTOR1; p18; p27kip1 releasing factor from RhoA; p27Kip1-releasing factor from RhoA; p27RF-Rho; PDRO; Protein associated with DRMs and endosomes; Ragulator complex protein LAMTOR1; ragulator complex protein PDRO; Ragulator1; RhoA activator C11orf59 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG