CST, 87795T, NEK7 (E4E1B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NEK7. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NEK7 (E4E1B) Rabbit Monoclonal Antibody (CST #87795) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NEK7 (E4E1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total NEK7 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NEK7 protein. Background NEK7 (NIMA-related protein kinase 7), is one of a family of serine/threonine kinases, named NEK1-NEK11, which are involved in the control of mitosis and other non-mitotic functions (1-6). NEK7 is the smallest NIMA-related kinase in mammals, and is widely expressed by many tissues (1-3). Its role in mitosis relates to centrosome enrichment and microtubule nucleation, and similar to other NIMA-related kinases, NEK7 is localized to the microtubule-organizing center (MTOC) at the time of mitotic entry (3,6). NEK7 has also been demonstrated to be essential for NLRP3 inflammasome activation by directly binding to the leucine-rich repeat (LRR) domain of NLRP3 (4-6). Alternate Names NEK7; Never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related kinase 7; NIMA related kinase 7; NimA-related protein kinase 7; Serine/threonine-protein kinase Nek7 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 32 Source/Isotype: Rabbit IgG