CST, 8779S, SirT5 (D5E11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SirT5. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, SirT5 (D5E11) Rabbit Monoclonal Antibody (CST #8779) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SirT5 (D5E11) Rabbit Monoclonal Antibody recognizes endogenous levels of total SirT5 protein. This antibody does not cross-react with other sirtuin proteins. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the full-length human SirT5 protein. Background The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT5, a mammalian homolog of Sir2, is localized to the mitochondria and has been implicated in the regulation of cell metabolism (2,3). SirT5 deacetylates carbamoyl phosphate synthetase 1 (CPS1) in the mitochondrial matrix and increases its activity in response to fasting, allowing for adaptation to increased amino acid catabolism (4). SirT5 has also been shown to deacetylate cytochrome c in the mitochondrial intermembrane space (5). In addition to its deacetylase activity, SirT5 contains lysine desuccinylase and demalonylase activity (6,7). Succinyl-lysine and malonyl-lysine modifications occur in a variety of organisms and these post-translational modifications are found on many metabolic enzymes (6-8). Like phosphorylation of serine, threonine, and tyrosine residues, lysine succinylation and malonylation induces a change of two negative charges from a +1 to a -1 charge at physiological pH, and are thought to serve similar functions in the regulation of protein activity, protein-protein interactions, and protein stability. SirT5 knockout mice show increased levels of succinyl-lysine and malonyl-lysine protein modifications in the liver, including increased succinylation of CPS1, a known target of SirT5, suggesting that SirT5 functions to regulate metabolic enzymes through its deacetylase, desuccinylase, and demalonylase activities (6,7). Alternate Names FLJ36950; NAD-dependent deacetylase sirtuin-5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial; NAD-dependent protein deacylase sirtuin-5, mitochondrial; Regulatory protein SIR2 homolog 5; silent mating type information regulation 2, S.cerevisiae, homolog 5; sir2-like 5; SIR2-like protein 5; SIR2L5; SIR5; SIRT5; sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae); sirtuin 5; sirtuin type 5 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG