CST, 8780S, RanBP1 Antibody

Polyclonal Antibody for studying RanBP1. Validated for Western Blotting. Highly specific and rigorously validated in-house, RanBP1 Antibody (CST #8780) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RanBP1 Antibody recognizes endogenous levels of total RanBP1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr193 of human RanBP1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background RanBP1 is a Ran binding protein that functions in nuclear trafficking for both nuclear import and export (1-3). Its protein sequence contains a Ran binding domain and a C-terminal nuclear export signal, which maintains its cytoplasmic localization (2,3). During nuclear export, RanBP1 forms a complex with RanGTP and CRM1/cargo, leading to dissociation of cargo from CRM1 (2,4). RanBP1 further stimulates RanGTP-RanGAP1 association to facilitate RanGTP hydrolysis and the generation of RanGDP to complete the final steps of nuclear export (5). During nuclear import, RanBP1 stabilizes the formation of a RanGDP-importin/NLS receptor-RanBP1 complex. This complex regulates the release of imported cargo into the nucleus (6,7). In addition to nuclear trafficking, RanBP1 also controls RanGTP distribution along mitotic microtubules, which localizes critical factors, such as cyclin B1 and HURP, to mitotic microtubles and regulates chromosome segregation (8,9). knock down or overexpression of RanBP1 has been shown to affect cellular ciliogenesis by regulating the local RanGTP concentation at the base of cilia (10). Alternate Names HpaII tiny fragments locus 9A; HTF9A; MGC88701; RAN binding protein 1; Ran-binding protein 1; Ran-specific GTPase-activating protein; RANBP1; RANG; testis secretory sperm-binding protein Li 221n Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 27 SOURCE: Rabbit