CST, 87823SF, CD28 (D2Z4E) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD28. Validated for WB,IHC,IHC,IF. Highly specific and rigorously validated in-house, CD28 (D2Z4E) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #87823) is ready to ship. Product Usage Information This product is the carrier free version of product #38774. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 38774 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD28 (D2Z4E) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD28 protein and is predicted to react with multiple CD28 isoforms that are produced through alternative splicing. This antibody cross-reacts with 16 kDa and 100 kDa proteins of unknown origin in some mouse cells. Non-specific staining was observed in mouse testis by immunohistochemistry. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro208 of human CD28 protein. Background CD28 is a transmembrane glycoprotein expressed by T cells as well as some other hematopoietic cells (1,2). T cell activation requires T cell receptor (TCR) recognition of antigen presented in the context of MHC molecules. CD28 acts as a T cell costimulatory receptor, and interaction of CD28 with its ligands CD80 or CD86 provides the second signal required for naïve T cell activation (3-5). Activation of naïve T cells in the absence of CD28 stimulation can result in a state of T cell anergy, or unresponsiveness (3). CD28 signals through cytoplasmic phospho-tyrosine motifs that bind several SH2 or SH3 domain-containing proteins involved in T cell activation (2). Recently, CD28 was demonstrated to be a preferred target of PD-1-mediated dephosphorylation. Consistently, CD28 expression was required for T cell proliferation following PD-1 blockade and CD28 stimulation was required for effective anti-PD-1 cancer immunotherapy in mice (6,7). Several CD28 isoforms are produced by alternative splicing (8). Alternate Names CD28; CD28 antigen; CD28 molecule; MGC138290; T-cell-specific surface glycoprotein CD28; TP44 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 14, 40-60 Source/Isotype: Rabbit IgG