CST, 87933S, Phospho-PFAS (Thr619) Antibody

Polyclonal Antibody for studying PFAS (Thr619) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-PFAS (Thr619) Antibody (CST #87933) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PFAS (Thr619) Antibody recognizes endogenous levels of PFAS protein only when phosphorylated at Thr619. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr619 of human PFAS protein. Antibodies are purified by peptide affinity chromatography. Background Phosphoribosylformylglycinamidine synthase (PFAS) is a metabolic enzyme (deamidase) associated with a protein complex known as the "purinosome," which is involved in purine synthesis (1). Research studies have shown that activation of the MAPK/ERK pathway leads to downstream activation of PFAS. Specifically, it was shown that PFAS is a substrate of ERK2, which phosphorylates PFAS at Thr619; activation of PFAS results in the generation of purine intermediates required for cell and tumor growth (2). It has also been reported that both PFAS and viral PFAS-like proteins can activate RIG-I pattern recognition receptors, implicating PFAS-mediated deamidation in the innate immune response to viral infection (3). Alternate Names FGAM synthase; FGAMS; FGAR amidotransferase; FGAR-AT; FGARAT; Formylglycinamide ribonucleotide amidotransferase; Formylglycinamide ribotide amidotransferase; Formylglycinamide ribotide synthetase; GATD8; IKZF1/PFAS fusion; KIAA0361; PFAS; Phosphoribosylformylglycinamidine synthase; PUR4; PURL Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 145 SOURCE: Rabbit