CST, 87960S, CD23 (E1R4G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD23. Validated for Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD23 (E1R4G) Rabbit Monoclonal Antibody (CST #87960) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:800 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 89186 . Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity CD23 (E1R4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD23 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala118 of human CD23 protein. Background CD23, also known as FcεRII, is a trimeric type II transmembrane glycoprotein member of the calcium-dependent (C-type) lectin family (1). CD23 is expressed in B and T lymphocytes, monocytes, polymorphonuclear leukocytes, follicular dendritic cells, intestinal epithelial cells, and bone marrow stromal cells (2-8). CD23 was first identified as a low-affinity receptor for IgE and the engagement of membrane bound CD23 leads to suppressed IgE production by B lymphocytes (9). CD23 can be cleaved to form a soluble protein which can drive cytokine release in monocytic cells, nitric oxide (NO) production, and the synthesis of cyclic adenosine 3′5′-monophosphate (cAMP) (10). Soluble CD23 signals through integrins, activating MAPK and NF-κB pathways (11). CD23 is a useful marker in the prognosis of neoplastic disease, is elevated in a variety of autoimmune and inflammatory conditions, and is being investigated as a therapeutic target for IgE-mediated allergy, arthritis, and B cell chronic lymphocytic leukemia (CLL) (12). Alternate Names BLAST-2; C-type lectin domain family 4 member J; C-type lectin domain family 4, member J; CD23; CD23 antigen; CD23A; CLEC4J; Fc epsilon receptor II; Fc fragment of IgE receptor II; Fc fragment of IgE, low affinity II, receptor for (CD23); Fc-epsilon-RII; FCE2; FCER2; IGEBF; Immunoglobulin E-binding factor; immunoglobulin epsilon-chain; Low affinity immunoglobulin epsilon Fc receptor; Low affinity immunoglobulin epsilon Fc receptor membrane-bound form; Low affinity immunoglobulin epsilon Fc receptor soluble form; Lymphocyte IgE receptor Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG