CST, 88019T, METTL1 (E4Z5X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying METTL1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, METTL1 (E4Z5X) Rabbit Monoclonal Antibody (CST #88019) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity METTL1 (E4Z5X) Rabbit Monoclonal Antibody recognizes endogenous levels of total METTL1 protein. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala55 of human METTL1 protein. Background METTL1 is a conserved methyltransferase that forms a complex with WDR4 to catalyze 7-methylguanosine (m7G) on mRNA, tRNA, and miRNAs (1,2). Both METTL1 and WDR4 are required for m7G modification, with METTL1 being the catalytic component and WDR4 stabilizing the complex (2). m7G on mRNA is required for translation and stem cell differentiation, while m7G on miRNA can regulate structure and biogenesis (3,4). METTL1 is phosphorylated by PKB and RSK, which results in its inactivation (5). Overexpression of METTL1 has been shown to be oncogenic and contribute to poor prognosis in various tumor types (6-8). Alternate Names C12orf1; D1075-like gene product; FLJ95748; methyltransferase like 1; methyltransferase-like 1; Methyltransferase-like protein 1; METTL1; miRNA (guanine-N(7)-)-methyltransferase; mRNA (guanine-N(7)-)-methyltransferase; TRM8; TRMB; TRMT8; tRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase; YDL201w Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG