CST, 8802S, GRAF1 Antibody

Polyclonal Antibody for studying GRAF1. Validated for Western Blotting. Highly specific and rigorously validated in-house, GRAF1 Antibody (CST #8802) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GRAF1 Antibody recognizes endogenous levels of total GRAF1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys261 of human GRAF1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background GTPase Regulator Associated with Focal Adhesion Kinase-1 (GRAF1), is a GTPase-activating protein for the small G proteins RhoA and Cdc42 (1). It is composed of an N-terminal BAR domain, a PH domain, a RhoGAP domain, a proline-rich domain, and a C-terminal SH3 domain. GRAF1 contributes to the clathrin-independent carriers/GPI-enriched early endosomal compartments (CLIC/GEEC) pathway, and was the first specific protein component of this endocytic pathway to be discovered (2). GRAF1 was identified as an important protein necessary for adeno-associated virus 2 infection (3). In addition, research studies have linked GRAF1 to mental retardation (4), skeletal muscle differentiation (5), and myeloid leukemia (6,7). Alternate Names ARHGAP26; FLJ42530; GRAF; GRAF1; GTPase regulator associated with focal adhesion kinase; GTPase regulator associated with focal adhesion kinase pp125(FAK); KIAA0621; Oligophrenin-1-like protein; OPHN1L; OPHN1L1; RHG26; Rho GTPase activating protein 26; Rho GTPase-activating protein 26; Rho-type GTPase-activating protein 26 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 92 SOURCE: Rabbit