CST, 88073S, Human Erythropoietin Recombinant Protein

Growth Factors and Cytokines for studying EPO in the research area. Storage Human Erythropoietin Recombinant Protein is supplied as lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC. Source / Purification Recombinant hEPO was expressed in 293 cells and is supplied in a lyophilized form. Background Erythropoietin (EPO) is a 34 kDa protein that is required for the survival and terminal differentiation of erythrocytes (1). EPO is produced by several cell types, including tubular endothelial cells, interstitial cells, hepatocytes, Kupffer cells, astrocytes, and Schwann cells (2-4). EPO inhibits apoptosis and may protect neuronal cells from death during ischemia and/or neurodegenerative diseases (3,4). EPO inhibits pro-inflammatory cytokine production by inhibiting NF-κB signaling (5). In an animal model, EPO protected against the development of diabetes by promoting pancreatic β cell survival and growth (6). Binding of EPO to its cognate receptor, EPOR, induces activation of Jak2, Stat5, and Akt (3-5).