CST, 88077S, Phospho-LAT (Tyr161) (E9Q2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LAT (Tyr161) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-LAT (Tyr161) (E9Q2R) Rabbit Monoclonal Antibody (CST #88077) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-LAT (Tyr161) (E9Q2R) Rabbit Monoclonal Antibody recognizes endogenous levels of LAT protein only when phosphorylated at Tyr161. Tyr161 of LAT, long isoform corresponds to Tyr132 of LAT, short isoform. This antibody cross-reacts with phosphorylated EGFR. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr161 of human LAT protein, long isoform. Background LAT, a transmembrane adaptor protein expressed in T, NK, and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr220 enables the binding of Grb2, Gads/SLP-76, PLCγ1, and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4). Research studies have shown that Zap-70 phosphorylates LAT at Tyr161, which creates an additional docking site to facilitate PLCγ1 recruitment, phosphorylation, and activation (3-5). Tyr161 is a relatively poor substrate for Zap-70 and following TCR engagement, phosphorylation of LAT at Tyr161 is delayed relative to the other distal tyrosine residues. Given the importance of PLCγ1 in regulating multiple T cell effector functions, it is posited that the delayed kinetics of Tyr161 phosphorylation serves as molecular rheostat in order to allow the TCR to effectively discriminate between autoantigens and foreign antigens (6,7). Alternate Names 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; IMD52; LAT; LAT1; linker for activation of T cells; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36-38; pp36 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG