CST, 88084SF, Galectin-1/LGALS1 (8A12) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying galectin-1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Galectin-1/LGALS1 (8A12) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #88084) is ready to ship. Product Usage Information This product is the carrier free version of product #40103. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 40103 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Galectin-1/LGALS1 (8A12) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total galectin-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human galectin-1 protein. Background Galectins are a family of β-galactose binding proteins that are characterized by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and their carbohydrate-binding site (1,2). Members of the galectin family have been implicated in a variety of biological functions including cell adhesion (3), growth regulation (4), cytokine production (5), T cell apoptosis (6), and immune responses (7). Galectin-1/LGALS1 has been shown to be expressed in a wide range of tissues and cell types. The level and pattern of expression of galectin-1 have been shown to change during development (8). In addition to a role in developmental processes, galectin-1 has been shown to be involved in central immune tolerance and may function in tumorigenesis by modulating the immune response to the tumor (9,10). Research studies have shown that galectin-1 expression is increased in several human cancers, suggesting a correlation with metastatic potential (10). Alternate Names 14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; DKFZp686E23103; epididymis secretory sperm binding protein; Gal-1; GAL1; Galaptin; galectin 1; Galectin-1; GBP; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LEG1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Mouse IgG1