CST, 88086S, SARS-CoV-2 Nsp3 Antibody

Polyclonal Antibody for studying nsp3-pp1a-pp1ab SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 Nsp3 Antibody (CST #88086) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 Nsp3 Antibody recognizes endogenous levels of total SARS-CoV-2 Nsp3 protein in infected cells. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro395 of SARS-CoV-2 Nsp3 protein. Antibodies are purified by peptide affinity chromatography. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp3, also known as papain-like protease (PLpro), is the third protein to be translated from the viral RNA by the eukaryotic translational machinery. In a sequence of processing events, Nsp3 is cleaved from pp1a and pp1ab polyproteins, and works to also release 3C-like protease, a cysteine-like protease that performs subsequent polyprotein cleavage events that give rise to the components of the viral replicase complex (3). Both proteases are targets of interest for pharmaceutical anti-viral therapy (4,5). Alternate Names non-structural protein 3; non-structural protein 3 (819-2763); nsp3; nsp3-pp1a-pp1ab; Papain-like proteinase; PL-PRO; PL2-PRO; PLPro; SARS coronavirus main proteinase; SARS-CoV-2 nsp3 Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 150-250 SOURCE: Rabbit