CST, 88113T, VCre Recombinase (F3Q3G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying VCre recombinase. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, VCre Recombinase (F3Q3G) Rabbit Monoclonal Antibody (CST #88113) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity VCre Recombinase (F3Q3G) Rabbit Monoclonal Antibody recognizes transfected VCre recombinase protein. This antibody also detects a small subset of Cre positive cells by immunofluorescence. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of VCre recombinase protein. Background Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base-pair loxP sites. This reaction can be carried out , indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of and applications, including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6). Similar to Cre/Lox, two additional site-specific recombination systems have been identified: VCre/VloxP and SCre/SloxP. Although the structure of their recognition sites are identical, Cre, VCre, and SCre recombinases do not cross-react with each other, allowing for in tandem use (7). Alternate Names phage integrase family protein Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 42 Source/Isotype: Rabbit IgG