CST, 8811S, His-Tag (27E8) Mouse Monoclonal Antibody (Magnetic Bead Conjugate)

Monoclonal Antibody for studying . Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, His-Tag (27E8) Mouse Monoclonal Antibody (Magnetic Bead Conjugate) (CST #8811) is ready to ship. Product Usage Information Immunoprecipitation: 1:20 Storage Supplied in PBS Buffer (pH 7.2), 0.1% Tween ® 20. Store at 4°C. Do not aliquot the antibodies. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity His-Tag (27E8) Mouse Monoclonal Antibody (Magnetic Bead Conjugate) detects recombinant proteins containing the 6xHis epitope tag. This antibody conjugate recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse IgG1