CST, 88145S, hnRNP M (E3J6F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying hnRNP M. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, hnRNP M (E3J6F) Rabbit Monoclonal Antibody (CST #88145) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity hnRNP M (E3J6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total hnRNP M protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro500 of human hnRNP M protein. Background Heterogenous nuclear ribonucleoprotein M (hnRNP M) belongs to a family of RNA-binding multi-protein complexes (hnRNP proteins) that facilitate pre-mRNA processing (1,2). hnRNP M binds to GU rich regions of RNA and exists as 4 isoforms named M1-M4 (2). Binding to pre-mRNA in early stages of spliceosome assembly, hnRNP M contributes to splicing events by inducing exon skipping as well as promoting exon inclusion (3,4). hnRNP M is expressed on the surface of Kupffer cells, and acts as a receptor for carcinoembryonic antigen (CEA), a biomarker for colorectal cancer metastasis to the liver (5-7). hnRNP M has also shown to be a key regulator of the innate immune response in macrophages, where it represses key inflammatory and antimicrobial genes (8,9). Alternate Names CEA receptor; CEAR; DKFZp547H118; Heterogeneous nuclear ribonucleoprotein M; heterogenous nuclear ribonucleoprotein M; heterogenous nuclear ribonucleoprotein M4; hnRNA-binding protein M4; hnRNP M; HNRNPM; HNRNPM4; HNRPM; HNRPM4; HTGR1; M4 protein; N-acetylglucosamine receptor 1; NAGR1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG