CST, 88153S, VCIP135 Antibody

Polyclonal Antibody for studying VCIP135. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, VCIP135 Antibody (CST #88153) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity VCIP135 Antibody recognizes endogenous levels of total VCIP135 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1035 of human VCIP135 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, and JAMM. The valosin-containing protein p97/p47 complex-interacting protein 1 (VCIP135, VCPIP1) is a deubiquitinating enzyme that belongs to the A20-like subfamily of ovarian tumor (OTU) DUBs (3). VCIP135 serves as a cofactor for the p97/p47 complex in regulating Golgi membrane fusion and reassembly at the end of mitosis (4-6). Research studies suggest that the phosphorylation status of VCIP135 provides a mechanism to fine-tune the kinetics of Golgi disassembly and reassembly during the cell cycle. For example, these studies demonstrate that VCIP135 undergoes phosphorylation early in mitosis, which blocks its association with the Golgi membrane and p97/VCP, thus inhibiting p97/VCP-mediated Golgi membrane fusion (7,8). Alternate Names Deubiquitinating protein VCIP135; Deubiquitinating protein VCPIP1; DKFZp686G038; DUBA3; FLJ23132; FLJ60694; KIAA1850; valosin containing protein (p97)/p47 complex interacting protein 1; valosin containing protein interacting protein 1; valosin-containing protein (p97)/p47 complex-interacting protein p135; Valosin-containing protein p97/p47 complex-interacting protein 1; Valosin-containing protein p97/p47 complex-interacting protein p135; VCIP1; VCIP135; VCP/p47 complex-interacting 135-kDa protein; VCPIP1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 140 SOURCE: Rabbit