CST, 88188T, REST (E3L2I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying REST. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, REST (E3L2I) Rabbit Monoclonal Antibody (CST #88188) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity REST (E3L2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total REST protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human REST protein. Background Repressor element 1-silencing transcription factor (REST), also known as neural-restrictive silencer factor (NRSF), is a repressive transcription factor that acts by binding to neuron-restrictive silencer elements present in many neuronal genes (1,2). The C-terminal domain of REST is bound by its cofactor, CoREST, which in turn recruits many epigenetic regulators, including HDAC1/2, Sin3A, MeCP2, and LSD1 (3-5). REST is capable of local and long-range chromatin remodeling, including changes to nucleosome phasing and histone modifications (6,7). REST also plays a key role in miRNA regulation along with CREB through the control of genes involved in miRNA biogenesis (8). Many of the miRNAs regulated by REST exist in feedback loops, which control neural stem cell self-renewal and maintenance (9,10). REST also is capable of regulating many long non-coding RNAs (11). REST and its regulatory network of genes and RNAs have been implicated in numerous neurological disorders, including schizophrenia, Huntington's, and Alzheimer's disease (12-14). Alternate Names DFNA27; GINGF5; HGF5; Neural-restrictive silencer factor; neuron restrictive silencer factor; NRSF; RE1 silencing transcription factor; RE1-silencing transcription factor; repressor binding to the X2 box; REST; WT6; X2 box repressor; XBR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG