CST, 88194SF, DKK1 (D5V6L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying DKK1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, DKK1 (D5V6L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #88194) is ready to ship. Product Usage Information This product is the carrier free version of product #48367. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 48367 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity DKK1 (D5V6L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total DKK1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DKK1 protein. Background Dickkopf (DKK) family proteins consist of four members (DKK1, DKK2, DKK3, and DKK4) that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6). DKK1 was initially identified as an inducer of head formation in Xenopus embryos (7) and plays an important role in the regulation of bone mass (8-10). Research studies indicate that increased levels of DKK1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers (11,12), while DKK1 expression is decreased in malignant melanoma and colorectal cancers (13,14). Alternate Names dickkopf 1 homolog; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; dickkopf WNT signaling pathway inhibitor 1; Dickkopf-1; dickkopf-1 like; dickkopf-like protein 1; Dickkopf-related protein 1; Dkk-1; DKK1; hDkk-1; SK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28-40 Source/Isotype: Rabbit IgG