CST, 88224S, Phospho-PANK2 (Ser169) (F7O5Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PANK2 (Ser169) phosphate. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-PANK2 (Ser169) (F7O5Q) Rabbit Monoclonal Antibody (CST #88224) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PANK2 (Ser169) (F7O5Q) Rabbit Monoclonal Antibody recognizes endogenous levels of PANK2 protein only when phosphorylated at Ser169. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser169 of human PANK2 protein. Background Pantothenate kinase 2 (PANK2) catalyzes the first step in coenzyme A (CoA) biosynthesis (1,2). Human PANK2 protein is located in the mitochondria, and research studies have shown some mutations in human are linked to neurodegeneration (2). Alternatively, mouse PANK2 protein localizes in the cytosol, and defective PANK2 protein in mice leads to retinal degeneration (1,3). Studies show that PI3K/Akt signaling induces CoA biosynthesis. CoA is a key metabolic cofactor in many metabolic processes, including the tricarboxylic acid (TCA) cycle and fatty acid metabolism, and is synthesized from pantothenate, cysteine, and ATP. Purified Akt kinase phosphorylates PANK2 . This phosphorylation is abolished when Ser169 and Ser189 residues on PANK2 are replaced by amino acid alanine (4). Alternate Names C20orf48; FLJ17232; Hallervorden-Spatz syndrome; HARP; hPanK2; HSS; MGC15053; NBIA1; PANK2; pantothenate kinase 2; Pantothenate kinase 2, mitochondrial; pantothenate kinase-associated neurodegeneration; Pantothenic acid kinase 2; PKAN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG