CST, 88228S, Integrin alpha2 Antibody

Polyclonal Antibody for studying Integrin-alpha-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Integrin alpha2 Antibody (CST #88228) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Integrin alpha2 Antibody recognizes endogenous levels of total integrin α2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly545 of human integrin α2 protein. Antibodies are purified by peptide affinity chromatography. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Integrin alpha-2, also known as cluster of differentiation 49b, forms a heterodimer with integrin beta-1. This integral membrane receptor complex interacts with extracellular matrix molecules laminin, collagen, collagen C-propeptides, fibronectin, and E-cadherin (3-6). It can also act as a receptor for viral pathogens (7,8). Integrin alpha-2/beta-1 is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, and force generation and organization of newly synthesized extracellular matrix (3). Alternate Names alpha 2 subunit of VLA-2 receptor; BR; CD49 antigen-like family member B; CD49B; Collagen receptor; GPIa; HPA-5; human platelet alloantigen system 5; integrin alpha 2; Integrin alpha-2; integrin subunit alpha 2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); ITA2; ITGA2; platelet antigen Br; platelet glycoprotein GPIa; platelet glycoprotein Ia; Platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2; VLA-2 subunit alpha; VLAA2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 170 SOURCE: Rabbit