CST, 88244T, Rab35 (E6B2S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rab35. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Rab35 (E6B2S) Rabbit Monoclonal Antibody (CST #88244) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rab35 (E6B2S) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rab35 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp124 of human Rab35 protein. Background Rab35 belongs to the small GTPase superfamily. By interacting with its specific GEF or GAP, Rab35 regulates cargo-specific endocytosis at the vesicle recycling step or mediates exocytosis at the exosome docking/tethering step (1-4). During cytokinesis, Rab35 interacts with OCRL phosphatase and is essential for maintaining intercellular bridge stability and abscission by controlling the concentration of phosphatidylinositol 4,5-bisphosphate (PIP2) and SEPT2 localization at the intercellular bridge (5,6). Rab35 also plays a role in actin assembly and the recruitment of Cdc42 and Rac1 to the site of filopodium by its direct interaction with actin-bundling protein fascin and actin-binding protein connecdenn 3 (7-9). Alternate Names GTP-binding protein RAY; H-ray; RAB1C; RAB35; RAB35, member RAS oncogene family; Ras-related protein Rab-1C; ras-related protein rab-1c (GTP-binding protein ray); Ras-related protein Rab-35; RAY Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 24 Source/Isotype: Rabbit IgG