CST, 8824T, Myosin IIb (D8H8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Myosin IIb. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Myosin IIb (D8H8) Rabbit Monoclonal Antibody (CST #8824) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Myosin IIb (D8H8) Rabbit Monoclonal Antibody recognizes endogenous levels of total myosin IIb protein. Nucleolar background may be observed in Myosin IIb low to negative cell lines by immunofluorescence. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human myosin IIb protein. Background Nonmuscle myosin is an actin-based motor protein essential to cell motility, cell division, migration, adhesion, and polarity. The holoenzyme consists of two identical heavy chains and two sets of light chains. The light chains (MLCs) regulate myosin II activity and stability. The heavy chains (NMHCs) are encoded by three genes, , , and , which generate three different nonmuscle myosin II isoforms, IIa, IIb, and IIc, respectively (reviewed in 1). While all three isoforms perform the same enzymatic tasks, binding to and contracting actin filaments coupled to ATP hydrolysis, their cellular functions do not appear to be redundant and they have different subcellular distributions (2-5). The carboxy-terminal tail domain of myosin II is important in isoform-specific subcellular localization (6). Research studies have shown that phosphorylation of myosin IIa at Ser1943 contributes to the regulation of breast cancer cell migration (7). Alternate Names Cellular myosin heavy chain, type B; cellular myosin heavy chain, type B type B; MGC134913; MGC134914; MYH10; Myosin heavy chain 10; Myosin heavy chain, non-muscle IIb; myosin heavy chain, nonmuscle type B; Myosin-10; myosin, heavy chain 10, non-muscle; myosin, heavy polypeptide 10, non-muscle; near to the ATP binding region; NMMHC II-b; NMMHC-B; NMMHC-IIB; NMMHCB; Non-muscle myosin heavy chain B; Non-muscle myosin heavy chain IIb; nonmuscle myosin heavy chain IIB; nonmuscle myosin heavy chain-B; nonmuscle myosin II heavy chain-B Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 230 Source/Isotype: Rabbit IgG