CST, 8829S, SMARCC2/BAF170 Antibody

Polyclonal Antibody for studying SMARCC2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SMARCC2/BAF170 Antibody (CST #8829) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SMARCC2/BAF170 Antibody recognizes endogenous levels of total SMARCC2/BAF170 protein (both isoforms 1 and 2). Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala223 of human SMARCC2/BAF170 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). SMARCC2/BAF170 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by Brg1 (10). While SMARCC2/BAF170 has been shown to be part of the SWI/SNF complex in non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Expression of SMARCC2/BAF170 has been shown to be up-regulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of SMARCC2/BAF170 leads to loss of stem cell pluripotency and self renewal (11). Alternate Names BAF170; BRG1-associated factor 170; chromatin remodeling complex BAF170 subunit; CRACC2; CSS8; DKFZp313D0632; mammalian chromatin remodeling complex BRG1-associated factor 170; Rsc8; SMARCC2; SMRC2; SWI/SNF complex 170 kDa subunit; SWI/SNF complex subunit SMARCC2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 2; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2; SWI3-like protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 162, 170 SOURCE: Rabbit