CST, 88300S, Integrin beta8 (D1V7M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Integrin beta 8 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin beta8 (D1V7M) Rabbit Monoclonal Antibody (CST #88300) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Integrin beta8 (D1V7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total integrin β8 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant mouse integrin β8 protein. Background Integrin αvβ8 facilitates activation and release of TGF-β, which has immunosuppressive effects (1). Deletion of integrin β8 from dendritic cells led to development of inflammatory bowel disease and autoimmunity in mice, as well as failure to induce regulatory T cells that require TGF-β for development (2). TGF-β is also involved in Th17 cell differentiation. Mice lacking expression of integrin β8 on dendritic cells fail to develop Th17 cells and are protected from Th17-mediated autoimmune disease (3). Regulatory T cells express high levels of integrin αvβ8, which enables them to suppress pathogenic T cell activation by activating latent TGF-β (4). Integrin β8 is also essential for vascular morphogenesis as integrin β8-deficient mice are embryonic or perinatal lethal and exhibit insufficient and abnormal vascular morphogenesis (5). Alternate Names 4832412O06Rik; Integrin beta; integrin beta 8; Integrin beta-8; ITB8; Itgb8 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 98 Source/Isotype: Rabbit IgG