CST, 88405SF, WT1 (D8I7F) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying WT1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, WT1 (D8I7F) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #88405) is ready to ship. Product Usage Information This product is the carrier-free version of product #83535. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 83535 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity WT1 (D8I7F) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total WT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human WT1 protein. Background Wilms' Tumor 1 (WT1) is a transcription factor named from Wilms' Tumor 1, an embryonal malignancy of the kidneys that is caused by mutations in the gene (1). It is highly important in development, particularly of the genitourinary system, and mutations and dysregulation of WT1 expression result in a variety of syndromes affecting the genitourinary system and other tissues (2-5). WT1 has a myriad of biological functions and a host of interacting partners and target genes (6). It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor (7). It exerts influence over the epigenetic landscape, and also has post-translational influence of gene expression through RNA interactions (8). The diverse biological roles of WT1 have been attributed to the existence of multiple isoforms and post-translational modifications of the protein (9). Alternate Names AWT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms tumor protein; WIT-2; WT1; WT1 transcription factor; WT33 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 51-60 Source/Isotype: Rabbit IgG