CST, 88428S, CDK4 (F4A7V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CDK4 mouse. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CDK4 (F4A7V) Rabbit Monoclonal Antibody (CST #88428) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CDK4 (F4A7V) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDK4 protein. Based on the sequence of the peptide antigen, this antibody is not expected to detect other CDK proteins. This antibody detects an approximately 15 kDa protein of unknown identity in some cell lines. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro254 of mouse CDK4 protein. Background Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle (4). Alternate Names Cdk4; Cell division protein kinase 4; Crk; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; p34/cdk4; PSK-J3; serine/threonine kinase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG