CST, 88436T, ITM2C/Bri3 (F3X8Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ITM2C. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, ITM2C/Bri3 (F3X8Z) Rabbit Monoclonal Antibody (CST #88436) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 - 1:400 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity ITM2C/Bri3 (F3X8Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ITM2C/Bri3 protein. This antibody detects full-length ITM2C/Bri3 (~31 kDa) and processed ITM2C/Bri3 without the C-terminal peptide (~27 kDa) by western blot. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ITM2C/Bri3 protein. Background The gene family comprises at least three members, BRI1/ITM2A, BRI2/ITM2B, and BRI3/ITM2C, which encode three type II transmembrane glycoproteins BRI1, BRI2, and BRI3, respectively. BRI3 expression is highest in the brain but the protein is also expressed in plasmacytoid dendritic cells, appendix, peripheral blood leukocytes, bone marrow, and fetal liver (1). BRI2 and BRI3 have been reported to negatively regulate amyloid β (Aβ) production by binding to Aβ precursor protein (APP) and inhibiting its processing by secretases. Both proteins mask the cleavage sites of α- and β-secretase on APP. However, unlike BRI2, the binding of BRI3 to the β-secretase cleaved APP C-terminal fragment is negligible (2,3). More recently, the gene signature has been identified as a potential marker for the early detection of colorectal cancer (4). Alternate Names BRI3; BRICD2C; BRICHOS domain containing 2C; Cerebral protein 14; CT-BRI3; E25; E25C; hRPC.1050_D_4; Integral membrane protein 2C; integral membrane protein 3; ITM2C; ITM3; Transmembrane protein BRI3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 31, 27 Source/Isotype: Rabbit IgG