CST, 88499S, PDE12 (E2V7J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PDE12. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PDE12 (E2V7J) Rabbit Monoclonal Antibody (CST #88499) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PDE12 (E2V7J) Rabbit Monoclonal Antibody recognizes endogenous levels of total PDE12 protein. This antibody detects precursor and mature isoforms of PDE12 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PDE12 protein. Background 2',5'-phosphodiesterase 12 (PDE12) is a ubiquitously expressed enzyme localized to the mitochondrial matrix. The catalytic activity of PDE12 is, in part, responsible for degradation of 2',5'-oligoadenylate second messengers (1-4). Through its ability to degrade 2',5'-oligoadenylates, PDE12 is considered to be a negative regulator of the interferon-induced OAS/RNase L antiviral signaling pathway. Indeed, either inhibition of PDE12 activity or loss of PDE12 expression has been shown to protect cells from viral pathogens by upregulating the activity of the OAS/RNase L pathway (1). Alternate Names 2-PDE; 2'-PDE; 2'-phosphodiesterase; 2',5'-phosphodiesterase 12; Mitochondrial deadenylase; PDE12; phosphodiesterase 12 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 75, 70 Source/Isotype: Rabbit IgG