CST, 8858S, PRAS40 (D23C7) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying PRAS40. Validated for Western Blotting. Highly specific and rigorously validated in-house, PRAS40 (D23C7) Rabbit Monoclonal Antibody (Biotinylated) (CST #8858) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PRAS40 (D23C7) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of total PRAS40 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro185 of human PRAS40 protein. Background Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5). Alternate Names 40 kDa proline-rich AKT substrate; AKT1 substrate 1; AKT1 substrate 1 (proline rich); AKT1S1; AKTS1; Lobe; PRAS40; Proline-rich AKT1 substrate 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG