CST, 8863S, Jak3 (D7B12) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Jak3. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Jak3 (D7B12) Rabbit Monoclonal Antibody (CST #8863) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Jak3 (D7B12) Rabbit Monoclonal Antibody recognizes endogenous levels of total Jak3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Jak3 protein. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Jak3 is primarily expressed in hematopoietic cells and is required for immune cell function and development (6-8). It binds to the common γ subunit (γc), a shared receptor subunit also used by several cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15 (9). IL-2 signaling and Stat5 activation are highly impaired by the loss of Jak3 (10,11). Jak3 is phosphorylated at multiple sites, including Tyr980 and 981 within the activation loop (12-14). Alternate Names JAK-3; JAK3; JAK3_HUMAN; JAKL; Janus kinase 3; Janus kinase 3 (a protein tyrosine kinase, leukocyte); L-JAK; Leukocyte janus kinase; LJAK; Tyrosine-protein kinase JAK3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 115 Source/Isotype: Rabbit IgG