CST, 88644S, Tapasin Antibody

Polyclonal Antibody for studying TAPBP. Validated for Western Blotting. Highly specific and rigorously validated in-house, Tapasin Antibody (CST #88644) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Tapasin Antibody recognizes endogenous levels of total Tapasin protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Tapasin protein. Antibodies are purified by peptide affinity chromatography. Background TAP-associated glycoprotein (Tapasin/TAPBP) is a type-I transmembrane ER glycoprotein encoded by an MHC-linked gene. Tapasin plays a critical role in the MHC class I-restricted antigen processing and presentation pathway. Research studies have demonstrated that Tapasin is an IFNγ-inducible component of the TAP-1/2 peptide transporter complex and, within the ER lumen, facilitates the optimization of the peptide repertoire that is loaded onto MHC class I molecules (1-5). Downregulation of Tapasin and MHC class I expression has been observed in human tumors and serves as a prominent mechanism of tumor immune escape (6-9). Alternate Names NGS-17; NGS17; TAP binding protein; TAP binding protein (tapasin); TAP-associated protein; TAP-binding protein; TAPA; Tapasin; TAPBP; TPN; TPSN Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 48 SOURCE: Rabbit