CST, 88650P, MDM2 (E3G5I) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying MDM2. Validated for Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, MDM2 (E3G5I) Rabbit Monoclonal Antibody (Biotinylated) (CST #88650) is ready to ship. Product Usage Information Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution. Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Specificity / Sensitivity MDM2 (E3G5I) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total MDM2 protein. Based on the sequence of the epitope, this antibody is expected to detect full-length MDM2, as well as isoforms A, C, alpha, F, G, and 11. This antibody may detect isoform A1. This antibody cross-reacts with a band of unknown origin at approximately 60 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human MDM2 protein. This antibody recognizes an epitope surrounding Val280 of human MDM2 protein. Background MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3). Alternate Names ACTFS; Double minute 2 protein; double minute 2, human homolog of; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; LSKB; MDM2; MDM2 oncogene, E3 ubiquitin protein ligase; Mdm2 p53 binding protein homolog (mouse); MDM2 proto-oncogene; MDM2 proto-oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; p53-binding protein; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2; ubiquitin-protein ligase E3 Mdm2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG