CST, 88652S, DJ-1 (D29E5) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying DJ-1. Validated for Western Blotting. Highly specific and rigorously validated in-house, DJ-1 (D29E5) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #88652) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity DJ-1 (D29E5) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of total DJ-1 protein. Species Reactivity: Human, Mouse, Rat, Hamster, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein. Background Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6). Alternate Names DJ-1; DJ1; epididymis secretory sperm binding protein Li 67p; FLJ27376; FLJ34360; FLJ92274; GATD2; HEL-S-67p; Maillard deglycase; Oncogene DJ1; PARK7; Parkinson disease (autosomal recessive, early onset) 7; Parkinson disease protein 7; parkinson protein 7; Parkinsonism associated deglycase; Parkinsonism-associated deglycase; protein deglycase DJ-1; Protein DJ-1; Protein/nucleic acid deglycase DJ-1 Specification REACTIVITY: H M R Hm Mk SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG