CST, 8865S, p21 Waf1/Cip1 (12D1) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying p21 Waf1/Cip1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, p21 Waf1/Cip1 (12D1) Rabbit Monoclonal Antibody (PE Conjugate) (CST #8865) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity p21 Waf1/Cip1 (12D1) Rabbit Monoclonal Antibody (PE Conjugate) detects endogenous levels of total p21 protein. This antibody conjugate does not cross-react with other CDK inhibitors. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p21 protein. Background The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5). Alternate Names CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDKN1A; CDN1A; CIP1; cyclin dependent kinase inhibitor 1A; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; MDA6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1; WAF1; wild-type p53-activated fragment 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG