CST, 8866S, G6PD Antibody

Polyclonal Antibody for studying G6PD. Validated for Western Blotting. Highly specific and rigorously validated in-house, G6PD Antibody (CST #8866) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity G6PD Antibody recognizes endogenous levels of total glucose-6-phosphate dehydrogenase protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human glucose-6-phosphate dehydrogenase protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first, and rate-limiting, step of the pentose phosphate pathway (1). The NADPH generated from this reaction is essential to protect cells from oxidative stress (1). Research studies have shown that p53 interacts with G6PD and inhibits its activity, therefore suppressing glucose consumption through the pentose phosphate pathway (2). In cancer cells with p53 mutations, the increased glucose consumption is directed towards increased biosynthesis, which is critical for cancer cell proliferation (2). Alternate Names epididymis secretory sperm binding protein; G6PD; G6PD1; Glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase; glucose-6-phosphate dehydrogenase, G6PD Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 58 SOURCE: Rabbit