CST, 88686S, MUC2 (CCP58) Mouse Monoclonal Antibody

Monoclonal Antibody for studying MUC2. Validated for IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, MUC2 (CCP58) Mouse Monoclonal Antibody (CST #88686) is ready to ship. Product Usage Information IHC Leica Bond: 1:800 - 1:3200 Immunohistochemistry (Paraffin): 1:800 - 1:3200 Storage Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 36 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles . A slight precipitate may be present, but will not interfere with antibody performance. Protocol Available protocols: IHC Leica Bond, Immunohistochemistry (Paraffin) Specificity / Sensitivity MUC2 (CCP58) Mouse Monoclonal Antibody recognizes endogenous levels of total MUC2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide from human MUC2 protein, coupled to KLH. Background Mucins are a family of large glycoproteins that create the structural component of mucus. Mucus functions as a blockade against pathogenic invasion and physical injury to the respiratory, gastrointestinal, and urogenital tracts (1). Mucins create a protective layer for epithelial cells as either membrane-bound (MUC1, MUC3, MUC16, and MUC17) or secreted (MUC2, MUC5AC, and MUC19) proteins. MUC2, or mucin-2, the main O-glycosylated protein found in mucus, is secreted by goblet cells (2). The structure of MUC2 contains cysteine-rich N- and C-terminal domains along with a protein core made up of heavily O-glycosylated mucin domains. The high levels of O-glycosylation along with disulfide bonding make MUC2 resistant to proteolytic cleavage (3). Mucin expression and glycan structure changes occur in cancers of the intestine and impact the development and progression of these cancers (4). The presence of MUC2 in the mucous layer of the colon helps prevent ulcerative colitis (UC) by inhibiting the invasion of bacteria (5). Alternate Names Intestinal mucin-2; MLP; MUC-2; MUC2; mucin 2, intestinal/tracheal; mucin 2, oligomeric mucus/gel-forming; Mucin-2; mucin-like protein; SMUC Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): >250 Source/Isotype: Mouse IgG1 kappa