CST, 88687SF, LAMP3/CD208 (E6E5U) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LAMP3. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, LAMP3/CD208 (E6E5U) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #88687) is ready to ship. Product Usage Information This product is the carrier free version of product #47778. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 47778 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity LAMP3/CD208 (E6E5U) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total LAMP3 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro257 of human LAMP3/CD208 protein. Background Lysosome-associated membrane proteins (LAMPs) are a family of proteins present predominantly on the membrane of lysosomes. Five members have been identified so far (LAMP1, LAMP2, LAMP3, CD68/Macrosialin/LAMP4, and BAD-LAMP/LAMP5). LAMP proteins are involved in many different aspects of cell biology and can influence cellular processes, such as phagocytosis, autophagy, lipid transport, and aging. Their expression varies among different tissues (1). LAMP3, also known as CD-LAMP and CD208, is highly expressed only in certain cell types and differentiation stages. LAMP3 is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation, but is not expressed by DCs in mouse (2-4). Its expression is associated with poor prognosis of certain tumors, and the locus where it is encoded was identified as a risk factor for Parkinson's disease (PD) (5). Alternate Names CD208; DC LAMP; DC-LAMP; DC-lysosome-associated membrane glycoprotein; DCLAMP; LAMP; LAMP-3; LAMP3; lysosomal associated membrane protein 3; Lysosomal-associated membrane protein 3; Lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 70, 140 Source/Isotype: Rabbit IgG