CST, 88701T, ORF1p (D3W9O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying L1RE1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, ORF1p (D3W9O) Rabbit Monoclonal Antibody (CST #88701) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ORF1p (D3W9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total ORF1p protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly162 of human ORF1p protein. Background LINE-1, also known as L1, is a non-long terminal repeat (non-LTR) retrotransposon with hundreds of thousands of copies in the human genome (1, 2). Like all non-LTRs, L1 replicates by target-primed reverse transcription (TPRT) (3). The L1 retrotransposon encodes two proteins critical to this process: ORF1p and ORF2p. ORF2p contributes to endonuclease and reverse transcriptase activity, while ORF1p acts as a nucleic acid chaperone that binds RNA (4-8). Many types of cancers have been shown to have L1 insertions within tumor suppressor genes, disrupting their expression and contributing to tumorigenesis (9). While normally silenced, ORF1p is expressed in many types of cancers (9-12). Recently, it has been found that ORF1p can become phosphorylated and that this event impacts its ability to promote retrotransposition. This creates a novel dynamic to which the host could actively affect L1 replication through various signaling cascades (13). Alternate Names L1ORF1p; L1RE1; LINE retrotransposable element 1; LINE-1 retrotransposable element ORF1 protein; LINE1 retrotransposable element 1; LORF1; LRE1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG