CST, 88742T, Osteopontin/SPP1 (E9Z1D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying osteopontin mouse. Validated for WB,WB,IP,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Osteopontin/SPP1 (E9Z1D) Rabbit Monoclonal Antibody (CST #88742) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Frozen): 1:200 - 1:800 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 17090 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Osteopontin/SPP1 (E9Z1D) Rabbit Monoclonal Antibody recognizes endogenous levels of total osteopontin/SPP1 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala149 of mouse osteopontin/SPP1 protein. Background Osteopontin is a highly phosphorylated secreted glycoprotein expressed in a variety of cell types. Osteopontin, encoded by the gene secreted phosphoprotein 1 (SPP1), was originally isolated and cloned from a rat osteosarcoma cell line (1). Osteopontin is a highly negatively charged protein that likely regulates extracellular matrix turnover and remodeling, both in bone and other tissue (2). Osteopontin also has three highly conserved receptor-binding domains, otherwise known as arginine-glycine-aspartic acid (RGD) domains. Binding of osteopontin to cell surface integrins likely activates cell signaling pathways that regulate cell proliferation, adhesion, invasion, migration, and fibrosis (3,4). Osteopontin may be regulated by posttranslational regulation, including phosphorylation, glycosylation, and proteolytic cleavage (5). Osteopontin is also linked to several human diseases. For example, cleaved osteopontin is higher in patients suffering with cirrhosis, a disease involving fibrosis of the liver (5). Its role in the brain is unclear, but SPP1 expression in microglia, the resident macrophages of the brain, is upregulated in mouse neurodegenerative disease models, including Alzheimer's disease (6). Osteopontin's role in disease likely involves its function as a key mediator of inflammatory responses (4). Alternate Names 2AR; 44 kDa bone phosphoprotein; AA960535; AI790405; Apl-1; BNSP; Bone sialoprotein 1; Bsp; BSPI; Calcium oxalate crystal growth inhibitor protein; Early T-lymphocyte activation 1 protein; Eta; ETA-; Eta-1; Minopontin; Op; Opn; Opnl; ost; Osteopontin; osteopontin-like protein; OSTP; OTTMUSP00000029095; OTTMUSP00000029097; OTTMUSP00000029098; Ric; Secreted phosphoprotein 1; SPP-1; Spp1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 58-75 Source/Isotype: Rabbit IgG