CST, 88831S, Calbindin (D1I4Q) Rabbit Monoclonal Antibody (Alexa Fluor® 594 Conjugate)

Monoclonal Antibody for studying Calbindin. Validated for Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Calbindin (D1I4Q) Rabbit Monoclonal Antibody (Alexa Fluor^® 594 Conjugate) (CST #88831) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity Calbindin (D1I4Q) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse calbindin protein. Background Calcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin, and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2). Alternate Names CAB27; CALB; CALB1; Calbindin; calbindin 1; calbindin 1, (28kD); calbindin 1, 28kDa; calbindin 27; Calbindin D28; D-28K; RTVL-H protein; Vitamin D-dependent calcium-binding protein, avian-type Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG